Figure 4.

Invasion assay of GES-1 gastric epithelial cells overexpressing mt or wt PTEN constructs following incubation with H. pylori. (A) Representative micrographs showing the Transwell invasion of hematoxylin-stained GES-1 cells expressing wt or mt PTEN constructs (GES-1-WT PTEN and GES-1-MT PTEN, respectively), with and without incubation with H. pylori (multiplicity of infection, 50; scale bar, 200 µm). (B) Numbers of cells invaded through the membranes are presented as the mean ± standard deviation of triplicated experiments. *P<0.05; **P<0.01; ***P<0.001. PTEN, phosphatase and tensin homolog; p-, phosphorylated; FAK, focal adhesion kinase; H. pylori, Helicobacter pylori; mt, mutant; wt, wild type.