Table 1.
GSH parameters measured in exponentially growing Wt and Δglr1 cells.
| [GSx] (mM)1 | [GSH] (mM) | [GSSG] (mM) | EGSH (mV) | [GSH]/[GSSG] | ||
|---|---|---|---|---|---|---|
| Wt | ER2 | 30 ± 2 | 29.6 ± 2 | 0.60 ± 0.04 | −242 ± 2 | 42 ± 2 |
| TotCell3 | 7 ± 1 | 6.6 ± 1 | 0.40 ± 0.05 | −2896 | NA7 | |
| Cor.TotCell4 | 31. 5 ± 4.5 | 29.7 ± 4.5 | NA8 | −2896 | NA | |
| Δglr1 | ER2 | 31 ± 1 | 29 ± 1 | 1.20 ± 0.03 | −235 ± 3 | 22 ± 2 |
| Cytosol5 | 31 ± 1 | 30 ± 1 | 0.50 ± 0.03 | −246 ± 2 | 46 ± 3 | |
| TotCell3 | 7.14 ± 1 | 6.44 ± 1 | 0.40 ± 0.05 | NA | NA | |
| Cor.TotCell4 | 32.13 ± 4.5 | 28.98 ± 4.5 | 1.80 ± 0.23 | NA | NA |
1
[GSx] = [GSH] + 2[GSSG]
2
Calculated from data obtained using ER-rxYFP and ER-1-Cys-Grx1
3
Measured in total cell lysates by enzyme assay
4
4.5-fold corrected value of [GSx]TotCell (see text and supplemental Fig. S2).
5
Calculated from data obtained using Cyt-rxYFP and Cyt-1-Cys-Grx1
6
Established in (Ostergaard et al., 2004)
7
Non applicable
8
The correction cannot be applied to GSSG, as TotCell also contains the vacuolar GSSG