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. Author manuscript; available in PMC: 2018 Oct 19.
Published in final edited form as: Cell. 2017 Sep 21;171(3):522–539.e20. doi: 10.1016/j.cell.2017.08.032

Figure 1. Transcriptomic analysis of GABAergic PCPs.

Figure 1

(A) Schematic of 6 PCPs with characteristic innervation patterns.

(B) Molecular markers parsing cortical GABA neurons into 3 non-overlapping populations and 6 PCPs.

(C) Experimental workflow.

(D) Bioinformatics pipeline (left) and DE genes across PCPs (right).

(E) Validation of known PCP markers; uTPM: unique transcripts per million.

(F) Novel PCP markers.

(G) Pthlh mRNA (grey arrowheads) co-localizes with RFP-labeled CHCs (yellow arrowheads) shown by FISH (left). Serial 3D reconstruction shows >95% RFP cells are Pthlh+ (right).

(H) Cck+ CHCs labeled in Nkx2.1-Flp;CCK-Cre;Ai65 cortex. Red: RFP; blue: DAPI; arrow: CHC; arrowhead: CHC axon boutons.

Also see Figure S1, S2, Table S1 and Methods.