Fig. 2.
Expression of ml1419c from M. leprae in P. aeruginosa PAO1. Recombinant P. aeruginosa PAO1 containing ml1419c, ml1419cΔGGDEF or the pJN105 vector (VC) were grown in the presence (+) or in the absence (−) of 0.2 % l- arabinose. Whole cell lysates (5 µg) of the recombinant strains were analysed by SDS-PAGE with Coomassie blue staining (a) and Western blot (b). Purified recombinant ML1419c produced in E. coli was used as a positive control (lane P) for Coomassie blue staining (500 ng) and Western blot (25 ng).