Table 1. Strains and plasmids used in the study.
| Strain or plasmid | Relevant genotype* | Source or reference† |
|---|---|---|
| BTH101 | cya99 araD139 galE15 galK16 rpsL1 (StrR) hsdR2 mcrA1 mcrB1 | Karimova et al. (2000) |
| BW25141 | lacIqrrnBT14 ∆lacZWJ16 ∆phoBR580 hsdR514 ∆araBADAH33 ∆rhaBADLD78 galU95 end-ABT333 uidA(∆MluI) :: pir+recA1 | Datsenko & Wanner (2000) |
| BW25311 | lacIqrrnBT14 ∆lacZWJ16 hsdR514 ∆araBADAH33 ∆rhaBADLD78 | Datsenko & Wanner (2000) |
| MG1655 | F-λ-rph1 ilvG rfb-50 | Lab stock |
| MGZ84 | MG1655 ftsZ84 (Ts) leu :: Tn10 | Manjula Reddy (CCMB) |
| TB28 | MG1655 ∆lacIZYA :: frt | Bernhardt & de Boer (2004) |
| XL10-Gold | endA1 glv44 recA1 thi1 gyrA96 relA1 lac Hte ∆(mcrA)183 ∆(mcrCB-hsdSMR-mrr)173 F´ [proAB lacIq Z∆M15 Tn10 (TetR) amy CmR] | Agilent |
| AC01-18 | JD505 ∆clpX :: KanR | |
| AC01-20 | JD505 pClpXP | |
| AC01-22 | JD505 pBR322 | |
| AJ-S08 | MG1655 ∆clpA :: KanR | |
| HVF1 | MG1655 ∆sspB :: KanR | |
| HVF2 | HVF1 pBAD33-zapC | |
| JD407 | MG1655 ∆clpX :: KanR | |
| JD409 | MG1655 ∆clpP :: KanR | |
| JD436 | MG1655 pBAD33-zapC | |
| JD454 | MG1655 pBAD33-zapC L22P | |
| JD455 | MG1655 pBR322 | |
| JD456 | MG1655 pClpXP | |
| JD458 | JD436 pBR322 | |
| JD459 | JD436 pClpXP | |
| JD474 | MG1655 pBAD33-zapCDD | |
| JD476 | JD474 pBR322 | |
| JD479 | JD474 pClpXP | |
| JD505 | TB28 ∆zapC :: frt | |
| LT57 | TB28 pNG162-zapC | |
| MB04 | JD409 pBAD33-zapC | |
| MB06 | JD407 pBAD33-zapC | |
| MB10 | JD407 pBAD33-zapCDD | |
| MB13 | JD409 pBAD33-zapCDD | |
| MB94 | AJ-S08 pBAD33-zapC | |
| MB99 | JD505 ∆clpP :: KanR | |
| MB103 | JD505 ∆clpA :: KanR | |
| MB110 | MB99 pNG162-zapC | |
| MB115 | MG1655 ∆clpA :: <frt> | |
| MB143 | MB115 ∆clpX :: KanR | |
| MB146 | MB143 pBAD33-zapC | |
| MB181 | MG1655 zapCDD-KanR | |
| MB184 | MGZ84 ∆clpP :: KanR | |
| MB188 | MGZ84 ∆clpX :: KanR | |
| MB199 | MGZ84 zapCDD-KanR | |
| MB203 | MB181 pClpXP | |
| Plasmid | ||
| AC01-09 | pKT25-ZapC; KanR | |
| AC01-10 | pKT25-ZapCDD; KanR | |
| AC01-58 | pUT18C-ClpX; AmpR | |
| pBAD33 | pACYC184 ori; PBAD; CmR | Lab stock |
| pBR322 | pMB1 ori; AmpR TetR | Lab stock |
| pClpXP | pBR322 clpX clpP; native promoter; AmpR | Michael Maurizi (NIH) |
| pCP20 | pSC101 ori; cI875 repA(Ts) PR:: flp; AmpR CmR | Datsenko & Wanner (2000) |
| pDONR221 | ColE1 ori; KanR; Gateway Donor vector | Thermo Fisher |
| pKD4 | R6K ori; bla frt<kan>frt | Datsenko & Wanner (2000) |
| pKD46 | pSC101 oriTs; araC-ParaB gam bet exo; AmpR | Datsenko & Wanner (2000) |
| pKT25 | pACYC184/15A ori; Plac :: T25-MCS; KanR | Tom Bernhardt (HMS) |
| pNG162 | pSC101 ori; lacIQ P204; SpcR | Goehring et al. (2006) |
| pUT18C-DEST | pBR322 ori; Plac:: T18-MCS; AmpR | Scot Ouellette (USD) |
*The KanR cassette is flanked by frt sites for removal by FLP recombinase. A frt scar remains following removal of the cassette using FLP expressed from pCP20.
†This work unless otherwise mentioned.