Table 2. Cell division phenotypes of zapC, clpX, clpA, clpP and zapCDD mutants.
| Strain | Genotype | Length (µm)* | n† | |||
|---|---|---|---|---|---|---|
| Mean | sd | Min. | Max. | |||
| MG1655 | WT | 2.9 | 0.6 | 1.8 | 6.1 | 89 |
| JD505 | zapC | 4.2 | 1.7 | 1.2 | 29.0 | 1709 |
| JD407 | clpX | 2.5 | 0.7 | 1.5 | 9.7 | 555 |
| AJ-S08 | clpA | 3.1 | 0.8 | 1.8 | 7.4 | 1049 |
| JD409 | clpP | 3.1 | 0.8 | 1.3 | 8.2 | 587 |
| AC18 | zapC clpX | 4.4 | 1.7 | 1.5 | 20.1 | 1407 |
| MB103 | zapC clpA | 3.3 | 1.3 | 1.6 | 18.8 | 900 |
| MB99 | zapC clpP | 4.3 | 4.0 | 1.2 | 45.7 | 791 |
| MB110 | zapC clpP/pNG162-zapC‡ | 3.2 | 1.0 | 1.5 | 9.6 | 544 |
| MB181 | zapCDD | 3.7 | 1.3 | 1.4 | 12.4 | 1205 |
*Cells were grown in LB at 37 °C and harvested at OD600=0.6–0.8, immobilized on 1 % agarose pads, and measurements determined using ObjectJ as described. Min. and Max., minimum and maximum cell lengths in the population.
†n, total number of cells measured. Less than 100 cells were measured for WT as cell length numbers approximated those described previously under the same conditions.
‡Leaky expression of IPTG-inducible plasmid in pNG162-zapC was used.