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. 2016 Jun 1;162(6):909–920. doi: 10.1099/mic.0.000278

Table 3. Cell division phenotypes upon overexpression of ClpXP in WT, zapC and zapCDD mutants.

Strain Genotype* Length (µm)† n
Mean sd Min. Max.
JD455 WT/pBR322 3.2 0.8 1.4 6.1 525
JD456 WT/pClpXP 6.6 5.3 2.4 59.9 664
AC01-22 zapC/pBR322 4.0 1.4 1.9 18.0 730
AC01-20 zapC/pClpXP 6.2 7.5 1.5 89.6 687
MB203 zapCDD/pClpXP 3.3 0.8 1.7 6.0 449

*Constitutive expression of pClpXP from native promoter in a pBR322 vector backbone in LB at 37 °C

†Cells were harvested at OD600=0.6, immobilized on 1 % agarose pads, and measurements determined using ObjectJ as described. Min. and Max., minimum and maximum cell lengths in the population.

n, total number of cells measured.