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. 2017 Nov 15;15(1):863–870. doi: 10.3892/ol.2017.7426

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — PAB induced autophagy in MRC5 cells. (A) Treatment with PAB increased the number of monodansylcadaverine-stained dots detected in fluorescence microscopy. Scale bar, 90 µm. (B) Expression of LC 3 and Beclin-1. A western blot was performed at 36 h after PAB treatment. Histone H3 is included as a loading control. (C) At 36 h after PAB treatment, intracellular LC 3A, LC 3B and Beclin-1 mRNA levels were detected. The results, as mean ± standard deviation, were standardized to the expression of GAPDH mRNA and normalized to the control group. The results are representative of three independent experiments. PAB, pseudolaric acid B; LC 3, light chain 3; Con, control.