Figure 5.

Inhibiting autophagy had no effect on the aggregation of microtubules and reduced the extent of cell cycle arrest induced by PAB. (A) Tubulin distribution and nuclear alterations analysis of PAB-treated MRC5 cells, as detected with fluorescence microscopy following phalloidin-tetramethylrhodamine B isothiocyanate and Hoechst 33258 staining. Scale bar, 60 µm. (B) Cell cycle profiles as determined by flow cytometry at 36 h. (C) Histograms of the percentage of cells in each phase of the cell cycle. The data are means ± standard deviation. **P<0.01; ***P<0.001. The results are representative of three independent experiments. PAB, pseudolaric acid B; Con, control; 3MA, 3-methyladenine.