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. 2017 Nov 10;15(1):864–872. doi: 10.3892/etm.2017.5482

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Copyright: © Xiao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Ectopic overexpression and knockdown of SDHB in CRC HT-29 cells. Four shRNAs targeting SDHB and a scrambled siRNA were transfected into HT-29 cells The human SDHB open reading frame was inserted into an enhanced green fluorescent protein plasmid-C1 vector to construct SDHB overexpression vectors. (A) Fluorescent photographs indicate the transfection efficiency (magnification, ×400). (B) Western blot analyses were performed to detect SDHB protein expression in HT-29 cells. *P<0.05, **P<0.01 vs. control group. SDHB, succinate dehydrogenase-B; CRC, colorectal cancer; shRNA, short-hairpin RNA; Con, control; OV, SDHB expression vector; sh1, SDHB-homo-305; sh2, SDHB-homo-447; sh3, SDHB-homo-498; sh4, SDHB-homo-779.