Table 2.
Molecular characteristics of HPV DNA+ ESCC tissues
| Patient Number |
Patient Seropositivity |
HPV type DNA | HPV type RNAd |
p16INK4a IHC |
||
|---|---|---|---|---|---|---|
|
| ||||||
| BSGP5+6+- PCR/MPGa |
TS-E7- PCR/MPGb |
HPV16/18 qPCRc | ||||
| 1 | 16 E6 | − | 16 | − | − | + |
| 2 | 6 E6 | − | 16 | − | 16 | − |
| 3 | 45 E6 | − | 16 | − | − | − |
| 4 | 16 E2 | − | 16 | − | − | − |
| 5 | 18 E6 | 33 | − | invalid | − | − |
| 6 | 16 E6 | − | 35 | − | − | − |
| 7 | 16 E1 | 45 | − | − | − | − |
| 8 | 18 E6 | − | 11 | − | − | − |
| 9 | 16 E1 | 11 | − | − | − | − |
| 10 | 16 E1 | 70 | 16 | invalid | − | − |
“−“, negative finding; “+“, positive finding; invalid, negative for HPV and β-globin DNA.
Available for all currently defined 51 mucosal HPV types (12 HR-, 8 pHR- and 31 LR-HPV); amplicon size: 150 bp HPV, 208 bp β-globin (total 51×118 = 6,018 reactions).
Available for 21 mucosal HPV types (12 HR-, 7 pHR- and 2 LR-HPV); amplicon size: ~100 bp HPV (for HPV6, 11, 16, 18, 31, 33, 35, 52, 56 and 66), 117 bp β-globin (total 21×118 = 2,478 reactions). For other HPV types the amplicon size was as previously published by Gheit and colleagues.
Available for HPV16 and 18; amplicon size: 104 bp HPV16, 110 bp HPV18, 110 bp β-globin (total 2×118 = 236 reactions).
Available for 21 mucosotropic HPV types (12 HR-, 8 pHR- and LR-HPV11); amplicon size: 65–75 bp HPV, 81 bp ubiquitin C. All samples were tested for the presence of HPV16 E6*I mRNA and for the presence of E6*I mRNA of the HPV type defined by genotyping assays and/or serology (total 170 reactions). E.g. for Patient Number 5, the RNA sample was analyzed for the presence of HPV16, HPV18 and HPV33 E6*I mRNA, respectively. No mRNA assay was available for LR-HPV type 6.