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. 2017 Nov 9;15(1):998–1006. doi: 10.3892/ol.2017.7386

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Copyright: © Annovazzi et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Immunohistochemical characterization of microglia/macrophages. (A) Resident ramified microglia in the normal cortex, Iba1 staining, ×400. (B) Diffuse astrocytoma. Reactive ramified microglia in mild tumor infiltration, Iba1 staining, ×200. (C) Diffuse astrocytoma. Reactive ramified microglia in high tumor infiltration, Iba1 staining, ×200. (D) Diffuse astrocytoma. Intermediate and macrophagic forms, CD16 staining, ×200. (E) Oligodendroglioma. Cells with a macrophage-like aspect in solid tumor, CD68 staining, ×200. (F) Oligodendroglioma. Reactive ramified microglia and macrophagic forms, Iba1 staining, ×200. (G) Oligodendroglioma. CD163⁺ cells were mainly found around vessels, CD163 staining, ×200. (H) Oligodendroglioma. In MIA and HIA Iba1⁺ RM did not crowd around small vessels, Iba1 staining, ×200. (I) Anaplastic oligodendroglioma. Intermediate and macrophagic forms in solid tumor, Iba1 staining, ×200. (J) Anaplastic astrocytoma. Intermediate and macrophagic forms in solid tumor, CD163 staining, ×200. All stained with DAB. Iba1, allograft inflammatory factor 1; DAB, 3,3′-diaminobenzidine; CD, cluster of differentiation; MIA, mild infiltration area; HIA, high infiltration area; RM, reactive ramified microglia.