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. 2017 Nov 9;15(1):998–1006. doi: 10.3892/ol.2017.7386

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Copyright: © Annovazzi et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Immunohistochemical characterization of microglia/macrophages. (A) GB; High frequency of CD16⁺ intermediate forms in solid tumor, CD16 staining, ×200, DAB. (B) GB; Perivascular CD163⁺ macrophagic forms, CD163 staining, ×200, DAB. (C) GB; Iba1+ macrophagic forms, Iba1 staining, ×200, DAB. (D) GB; Passage from a solid tumor to a regressive area: CD163+ cells are rare in the former and strongly positive and frequent as macrophagic forms in the latter, CD163 staining, ×200, DAB. (E) GB; Crowding of GAMs in glomerular structures, Iba1 (DAB) and CD34 (RED) stainings, ×200. (F) GB; Macrophagic forms with c-MAF⁺ nuclei and CD163⁺ cytoplasms, c-MAF (DAB) and CD163 (RED) stainings, ×630. (G) GB; Perivascular CD45⁺ M forms, CD45 staining, ×200, DAB. (H) GB; Perivascular CD163⁺ M forms, CD163 staining, ×200, DAB. (I) GB; CD98⁺ macrophage-like cells, CD98 staining, ×400, DAB. CD, cluster of differentiation; DAB, 3,3′-diaminobenzidine; HPF, high power fields; GB, Glioblastoma; Iba1, allograft inflammatory factor 1; GAMs, glioma-associated microglia/macrophages.