Table I.
Sequence of the polymerase chain reaction primers for each gene.
| Gene | Primers | Sequences |
|---|---|---|
| FLT3-ITD | Forward | 5′-GCAATTTAGGTATGAAAGCCAGC-3 |
| Reverse | 5′-CTTTCAGCATTTTGACGGCAACC-3 | |
| FLT3-TKD | Forward | 5′-CCAGGAACGTGCTTGTC-3 |
| Reverse | 5′-TCAAAAATGCACCACAGTGAG-3 | |
| NPM1 | Forward | 5′-TTAACTCTCTGGTGGTAGAATGAA-3 |
| Reverse | 5′-CAAGACTATTTGCCATTCCTAAC-3 | |
| C-KIT | ||
| Exon 8 | Forward | 5′-CTCCCTGAAAGCAGAAAC-3 |
| Reverse | 5′-CAGAAAGATAACACCAAAATAG-3 | |
| Exon 17 | Forward | 5′-GCAAAGGCATATTAGGAACTC-3 |
| Reverse | 5′-GTTGTAGTAATGTTCAGCATACC-3 | |
| NRAS | ||
| Exon 2 | Forward | 5′-AGAACCAAATGGAAGGTCACA-3 |
| Reverse | 5′-TGGGTAAAGATGATCCGACA-3 | |
| Exon 3 | Forward | 5′-GCAATTTGAGGGACAAACCA-3 |
| Reverse | 5′-CCCTAGATTCTCAATGTCAAACAA-3 | |
| KRAS | ||
| Exon 2 | Forward | 5′-CTTAAGCGTCGATGGAGGAG-3 |
| Reverse | 5′-AGAATGGTCCTGCACCAGTAA-3 | |
| Exon 3 | Forward | 5′-TTTTTGAAGTAAAAGGTGCACTG-3 |
| Reverse | 5′-TGCATGGCATTAGCAAAGAC-3 | |
| JAK2 (V617F) | Forward | 5′-ATCTATAGTCATGCTGAAAGTAGGAGAAAG-3 |
| Reverse | 5′-CTGAATAGTCCTACAGTGTTTTCAGTTTCA-3 |
FLT3, Fms related tyrosine kinase 3; NPM1, nucleophosmin; JAK2, Janus kinase 2.