Figure 6.

Transfection of miR-370 mimics leads to a decrease of phosphorylated FOXO3a and accumulation of FOXO3a in the nuclei of U-251MG cells. Cells were transfected with miR-370 mimics or negative controls for 48 h. (A) The levels of phosphorylated FOXO3a and total FOXO3a were detected by western blot analysis. β-actin was used as internal reference. (B) Nuclear FOXO3a was determined by western blot analysis in miR-370 mimics- and negative control-transfected cells. Lamin B1 was used as internal reference gene in the experiment. Ctrl., control; FOX, forkhead box protein; miR, microRNA.