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. 2017 Nov 13;15(1):1105–1112. doi: 10.3892/etm.2017.5495

Figure 1.

Figure 1.

LMP1 enhanced the protein expression of the EBV lytic proteins BZLF1 and BMRF1. (A) B95.8, CNE1 and constructed CNE1-LMP1 cells were lysed and western blot analysis was performed to measure the protein levels of EBNA1 and LMP1 in each cell type. β-actin was used as the loading control. (B) The protein levels of LMP1 and the EBV lytic proteins BZLF1 and BMRF1 in CNE1-LMP1 and CNE1 cells were determined by western blot analysis and compared with β-actin levels. (C) The protein levels of the EBV lytic proteins were analyzed by densitometry and presented as a ratio relative to the loading control β-actin. (D) CNE1 cells were transiently transfected with different quantities of LMP1-expressing plasmid (0, 0.5 and 1 µg) for 24 h and western blot analysis was performed to measure the protein levels of BZLF1, BMRF1, LMP1 and β-actin. (E) The levels of LMP1, BZLF1 and BMRF1 protein estimated by densitometry were presented as a ratio relative to the loading control β-actin. Values were presented as the mean ± standard deviation of triplicate experiments. *P<0.05 and ***P<0.001. LMP1, latent membrane protein 1; lyLMP1, lytic latent membrane protein 1; EBV, Epstein-Barr virus.