Figure 2.

Reduced LMP1 expression induced by LMP1-specific DZ1 treatment was associated with downregulation of the EBV lytic proteins BZLF1 and BMRF1 in LMP1-positive cells. (A) CNE1-LMP1 and (B) B95.8 cells were transfected with DZ1 or control ODN, and subjected to western blot analysis. The protein levels of LMP1, BZLF1 and BMRF1 in CNE1-LMP1 and B95.8 cells were measured by densitometry and presented as a ratio relative to the loading control β-actin. Untreated cells were considered as controls and set as 1. Cells treated with ODN were used as transfection mimics. Values were presented as the mean ± standard deviation of triplicate experiments. **P<0.01 and ***P<0.001. LMP1, latent membrane protein 1; lyLMP1, lytic latent membrane protein 1; EBV, Epstein-Barr virus; DZ1, DNAzyme; ODN, oligonucleotide.