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. 2017 Nov 15;15(1):871–878. doi: 10.3892/ol.2017.7420

Figure 2.

Figure 2.

Effect of CITED2 silencing on the expression of macrophage chemotactic factors. mRNA expression in (A) orthotopic xenograft tumors and (B) cells in culture, as determined by reverse transcription-quantitative polymerase chain reaction. (C) Protein expression in the two groups, as determined by ELISA. (D) Localization of CITED2 to the CCL20 promoter, as assessed by a chromatin immunoprecipitation assay using anti-CITED2 or IgG antibodies (control) in MDA-MB-231 and MDA-MB-468 cells. *P<0.05; **P<0.01; ***P<0.001 vs. scramble group (A-C) and vs. IgG (D). CITED2, Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain-2; sh, short hairpin; CCL, C-C motif chemokine ligand; Ig, immunoglobulin; IL, interleukin; CX3CL, C-X3-C motif chemokine ligand.