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. 2017 Nov 17;15(1):908–916. doi: 10.3892/ol.2017.7434

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Copyright: © Sun et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — XN promote apoptosis of breast cells. (A) MCF-7 and MDA-MB-231 cells were harvested for apoptotic analysis using Annexin V-FITC staining. (B) Quantification of apoptosis. Data are presented as the mean ± SD error of the mean of three independent experiments. (C) Apoptosis associated proteins Bcl-2, Bcl-xL and caspase-3 and cleaved PARP were analyzed by western blotting. GAPDH was used as the control. FITC, fluorescein isothiocyanate; FITC-A, FITC-annexin; Ctrl, control; XN, xanthohumol; DAPT, Duration of Dual Antiplatelet Therapy; PI-A, propidium iodide annexin; Bcl-2, B cell lymphoma-2; Bcl-xL, B cell lymphoma extra 1; PARP, poly (ADP-ribose) polymerase.