Table I.
Gene | Upstream primer | Downstream primer |
---|---|---|
Hes1 | 5′-TTGGAGGCTTCCAGGTGGTA-3′ | 5′-GGCCCCGTTGGGAATG-3′ |
Hey1 | 5′-CGAGGTGGAGAAGGAGAGTG-3′ | 5′-CTGGGTACCAGCCTTCTCAG-3′ |
GAPDH | 5′-TCTCATCACCATCTTCCA-3′ | 5′-CATCACGCCACAGTTTCC-3′ |
Hes1, hairy enhancer of split 1; Hey1, Hes related family BHLH transcription factor with YRPW motif 1.