Table II.
Target | Antibody type/clone | Host | Antibody dilution | Manufacturer |
---|---|---|---|---|
PTEN (pan) | Monoclonal 138G6 | Rabbit IgG | 1:500 | Cell Signaling Technology, Inc. |
Phospho-Akt Thr308 | Monoclonal D52E6 | Rabbit IgG | 1:1,500 | Cell Signaling Technology, Inc., no. 13038 |
PhosphoPDK1 Ser241 | Monoclonal C49H2 | Rabbit IgG | 1:1,000 | Cell Signaling Technology, Inc., no. 3438 |
AKT, AKT serine/threonine kinase; PDK-1, 3-Phosphoinositide-dependent kinase 1; PTEN, phosphatase and tensin homolog deleted on chromosome 10.