CQ treatment induced the inhibition of the proliferation, migration and colony-formation ability of EC109. (A) Cell viability detected by an MTT assay, displaying the dose- and time-dependent effect of CQ treatment on EC109 cells. Effect of CQ treatment on cell migration illustrated by (B) a scratch assay (magnification, ×100) and (C) a Transwell assay (magnification, ×200). (D) The colony-formation ability of EC109 cells as evaluated on soft agar. Each experiment was performed three times, independently. *P<0.05 vs. CON (determined by unpaired t-test). CQ, chloroquine; CON, control.