Table 2.
Nucleotide sequences of specific primers
| Target genes | GenBank accession | Primer sequences (5′ to 3′) | PCR products, bp |
|---|---|---|---|
| Primers used for QRT-PCR: | |||
| BHMT | XM_414685.3 | F: TCTTCCTGAATTTCCCTT R: TGAACATCCCATCTAGTGA |
57 |
| GNMT | XM_015283546.1 | F: GGAGGAGGGCTTCCAAGTGA R: GCTCCAGCGTCAGCCAGTT |
140 |
| DNMT1 | NM_206952.1 | F: CGAGTGGGACGGCTTCTT R: AGGCGATAGGTGTCAGGGA |
144 |
| VTGII | NM_001031276.1 | F: GAATCCTTCTCGACAAGCCAG R: GCTTCAGCAAAGACGTTCCAG |
186 |
| ERα | NM_205183.2 | F: TAGTTCCGCTCTACGACCTCTT R: AGTTGGTTTCGGTTCTCCTCTT |
106 |
| ERβ | NM_204794.2 | F: AGCGTGTTATGGTCTGCTCC R: GCTCTTAGGCTGCTCTGCAT |
87 |
| GR | NM_001037826.1 | F: CTTCCATCCGCCCTTCA R: TCGCATCTGTTTCACCC |
203 |
| β-actin | NM_205518.1 | F: ATG GCTCCGGTATGTGCA A R: TGTCTTTCTGGCCCATACCAA |
120 |
| Primers used for Bisulfite sequencing of GR promoter: | |||
| Segment 1 | F: TGTTATTTTGTAGAAGTGGGTGTGTTAG R: TAAACAACTTCRCACAACCCATTC |
||
| Segment 2 | F: GAATGGGTTGTGYGAAGTTGTTTA R: TTAAAATAACCCRACCRAATACTCC |
||
| Segment 3 | F: GTAYGGAGTYGTTTTGTTGGTTG R: ACTATCCTCCAAACCCATCTAATACC |
||
| Segment 4 | F: TTATTTGTAAATGTGGGGTTTATTTATAG R: TATAAACACTCCCCAACTCTACTATAACC |
||