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Force-distance curves for the overlap regions of single skeletal myofibrils in various physiological states obtained by approaching the cantilever at various velocities. (A) Rigor, (B) contracting, (C) contracting(+BDM), (D) +AMPPNP and (E) relaxed myofibrils. The approach velocity of cantilever: (a) 0.1, (b) 18, and (c) 38 μm/s. The tip of cantilever touched the top surface of the overlap regions at small arrows. The concentrations of BDM and AMPPNP: 20 mM and 2.5 mM, respectively.
