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. 2018 Jan 18;13(1):e0190962. doi: 10.1371/journal.pone.0190962

Fig 5. Cytokine dependent decrease in vSMC viability after type I NKT cell activation.

Fig 5

Splenocytes were cultured in presence of type I NKT cell specific ligands α-GalCer or OCH (50, 100 and 200 ng/ml) for 48 hours. Subsequently, supernatant of the splenocytes was added to vSMCs for 72 hours and viability of the vSMCs was assessed by an MTT assay (A). In addition, supernatant of the α-GalCer (200 ng/ml) stimulated splenocytes was pre-incubated with α-IFN-γ antibodies (B) and supernatant of OCH (200 ng/ml) stimulated splenocytes with α-IL4 (C) and α-IL10 (D) antibodies prior to addition of the supernatant to vSMCs. All values are mean±SEM and statistical analysis was performed using one-way ANOVA. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.