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. 2018 Jan 17;11:44. doi: 10.1186/s13104-018-3145-8

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Muguga derived Tp1_214–224 tetramer staining of PBMC from the three A18+ animals. BE017, BE033, BE043 and the control animal (BE029), isolated at day 15 after immunization. PBMC were co-stained with anti-CD8 Ab-PerCP and Tp1_214–224-tetramer-PE. Initial gating was performed on the live lymphocyte fraction (not shown). Staining of the CD8⁺ cells by the tetramer is seen in the upper right quadrant (Q2). The animals from which the CD8⁺ cells were derived are indicated in the figure. The experiment was performed twice