Fig 2. Oxytocin downregulates OXTR in NPCS.

1.5 μg of total protein was analyzed with Protein Simple Wes™ automated Western blotting system. Goat anti-OXTR antibody was used at a dilution of 1:10. Human uterus lysate was used as positive control. Cropped representative pseudo-blots show the expression of OXTR (60 kDa), with GAPDH as the loading control (Fig 2A). Full-length pseudo-blots are presented in Supplementary S1 Fig. Compared to control treatment, 24 h of 100 nM oxytocin significantly downregulated OXTR protein by approximately 45% (*P = 0.01 by Student’s t test) (Fig 2B). Data expressed as mean ± S.E.M from three biologically independent NPC cultures.