Table 1. CD related genes.

RNA-seq analysis of cardiovascular disease related genes
	Female Fold Change (p-value, FDR)		Male Fold Change (p-value, FDR)
Gene	Aging	RLX	Aging	RLX
IL-1β	-1.24 (ns)	1.48 (ns)	-1.69 (0.02, ns)	-3.54 (<0.0001, <0.01) ✓✓
IL-6	1.82 (0.03, ns) ✓	-1.81 (0.04, ns) ✓	1.15 (ns)	-4.35 (<0.0001, 0.01) ✓✓
IL-6RA	1.09 (ns)	-1.20 (ns)	-1.10 (ns)	1.21 (ns)
CRP	Undetected	Undetected	Undetected	Undetected
TNFα	-1.63 (ns) ✓	2.67 (<0.01, ns) ✓	-1.87 (<0.01, ns) ✓	-1.82 (0.03, ns) ✓
NPPB	1.00 (ns)	1.28 (ns)	1.43 (0.04, ns) ✓	-1.84 (<0.0001, 0.01) ✓✓
NPPA	10.48 (<0.0001, <0.0001) ✓✓	-2.95 (0.01, ns) ✓	1.37 (ns)	-1.36 (ns)
GDF-15	-1.45 (0.07, ns)	1.37 (ns)	1.40 (ns)	-3.58 (<0.0001, 0.03) ✓✓
SPP1	2.64 (<0.01, ns) ✓	-2.24 (<0.01, ns) ✓	-1.10 (ns)	-3.42 (<0.01, ns) ✓
CST3	1.35 (0.01, ns)	-1.47 (0.04, ns)	1.02 (ns)	-1.46 (<0.01, ns)
IFNG	6.10 (<0.0001, <0.01) ✓✓	-3.25 (<0.01, ns) ✓	-2.48 (0.03, ns) ✓	1.09 (ns)
FGF-23	1.41 (ns)	-1.14 (ns)	-4.44 (<0.01, ns) ✓	1.28 (ns)
FABP4	1.25 (ns)	-1.09 (ns)	1.81 (<0.01, ns) ✓	-1.03 (ns)
CSTB	1.28 (0.02, ns)	-1.18 (ns)	1.39 (ns)	-1.47 (ns)
IL-1RA	1.78 (<0.01, ns) ✓	-1.32 (ns)	2.11 (0.04, ns) ✓	-2.12 (0.03, ns) ✓
TNF-R1	-1.34 (0.04, ns)	1.14 (ns)	-1.16 (ns)	-1.16 (ns)
TNF-R2	1.05 (ns)	-1.17 (ns)	-1.19 (ns)	1.18 (ns)
Plaur_1	1.2 (ns)	-1.6 (0.02, ns) ✓	-1.21 (ns)	-1.39 (ns)
Plaur_2	1.77 (ns)	-1.76 (ns)	1.85 (ns)	-5.56 (<0.01, ns) ✓
Plaur_3	2.65 (0.01, ns) ✓	-3.55 (<0.01, ns) ✓	-1.2 (ns)	-5.29 (0.01, ns) ✓
CSF-1	1.4 (<0.01, ns)	-1.18 (ns)	1.23 (ns)	1.21 (ns)
AM	1.42 (0.01, ns)	-1.25 (ns)	1.06 (ns)	-1.14 (ns)
TNNT2_1	-1.05 (ns)	1.21 (ns)	1.3 (ns)	-1.53 (<0.01, ns) ✓
TNNT2_2	-1.13 (ns)	1.2 (ns)	1.26 (ns)	-1.26 (ns)
LDLR	-1.91 (<0.01, 0.05) ✓✓	1.56 (0.01, ns) ✓	-2.52 (<0.01, ns) ✓	1.32 (ns)

FDR: False Discovery Rate. Multiple test correction technique used in RNA-sequencing data analysis. Underscores indicated gene isoform. Bold: at least one group shows FDR < 0.05. ns: not significant. Yellow,✓✓: FDR<0.05. Blue,✓: Fold Change > 1.5, p<0.05.