Table 2. Primers used for real-time quantitative RT-PCR.

Gene		Sequence (5’-3’)	Length (bp)	Accession number
TGFβ1	For	CCTGCTGAGGCTCAAGTTAAAAG	81	L34956
	Rev	CTGAGGTAGCGCCAGGAATC
EGF	For	CTATGGCCCTCAAGGATGGTG	125	NM_001003094
	Rev	GCAGCCTTGCTCTGTGTCCTTA
HGF	For	AAAGGAGATGAGAAACGCAAACAG	95	AB090353
	Rev	GGCCTAGCAAGCTTCAGTAATACC
PDGFβ	For	TACGAGATGCTGAGCGACCAC	112	NM_001003383
	Rev	ATCGGGTCAAATTCAGGTCCAA
VEGFA	For	TTGCTGCTCTACCTCCACCAT	64	NM_001003175
	Rev	TGTGCTCTCCTCCTGCCATAG
IGF2	For	GGAAGAGTGCTGTTTCCGTA	125	XM_546602
	Rev	GGGTATCTGGGGAAGTTGTC
MMP2	For	ACTTTGATGACGATGAGCTATGGA	73	XM_014109407
	Rev	CCGTCGGCATTCCCATACT
MMP9	For	CTGGAGAGCTGGACAAAACC	232	NM_001003219
	Rev	TACACGCGAGTGAAGGTGAG
GAPDHa	For	GATGGGCGTGAACCATGAG	131	NM_001003142
	Rev	TCATGAGGCCCTCCACGAT
GUSa	For	CCTCCTGCCGTATTACCCTTG	117	NM_001003191
	Rev	TCTGGACGAAGTAACCCTTGG
RPS5a	For	TCACTGGTGAGAACCCCCT	141	XM_533568
	Rev	CCTGATTCACACGGCGTAG

^a Reference genes. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was identified as the most stable reference gene for normalization of relative mRNA concentration using the basic GeNorm Visual application for Microsoft Excel.