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. 2018 Jan 18;14(1):e1006718. doi: 10.1371/journal.ppat.1006718

Fig 2. Hierarchical clustering of GPCR genes expressed in adult S. mansoni.

Fig 2

Shown are functional groups to which GPCRs were assigned according to their relative transcript levels in adult S. mansoni obtained by a recent RNA-seq study from Lu et al. [32]. The heat map shows relative gene expression in all samples, which was calculated by the Z-score method implemented in the heatmap.2 function of the gplots package. Darker color indicates higher expression across all samples, and lighter color indicates lower expression levels (Color Key). The sM-bM-sF group contains GPCR genes with a transcription bias towards males and pairing-inexperienced females, while the “whole worm” group displays a balanced transcription rate among both sexes independently of pairing. Instead of this, the bF and sT-bT groups contain GPCRs that are preferentially transcribed in reproductive organs, namely testis, ovary, or vitellarium. S.m. gene provides Smp numbers for the listed GPCRs of S. mansoni. Existing/missing orthologs in F. hepatica (F. h.) are indicated by +/- (see S1 Table for details). sM, pairing-inexperienced (single-sex) males; bM, pairing-experienced (bisex) males; sF, pairing-inexperienced (single-sex) females; bF, pairing-experienced (bisex) females; sT, testes from sM; bT, testes from bM; sO, ovaries from sF; bO, ovaries from bF.