Skip to main content
. 2018 Jan 5;14(1):e1006804. doi: 10.1371/journal.ppat.1006804

Fig 4. ToxR activates leuO transcription in response to malate.

Fig 4

(A) Malate quantification in WT and RND null mutant JB485 culture supernatants and cells following growth under AKI conditions for 3.5h. Malate was quantified as described in the methods. The reported results are the means ± SD of six biological replicates with each replicate consisting of two technical replicates. * P≤0.05. (B) The indicated V. cholerae strains bearing pXB266 (leuO-lacZ) were cultured under AKI conditions for 4h when malate was added to 0, 1 or 2 mM. The cultures were then incubated with shaking for an additional hour when β-galactosidase production was quantified. The data are the mean ±SD of 3 independent experiments. * P≤0.05 relative to the no malate control.