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. Author manuscript; available in PMC: 2019 Jan 16.
Published in final edited form as: Immunity. 2017 Nov 28;48(1):35–44.e6. doi: 10.1016/j.immuni.2017.11.013

Figure 3. Hyperactive stimuli induce inflammasome assembly within living macrophages.

Figure 3

(A) Confocal imaging in the red and green channels of live cell ASC-citrine expressing BMDMs primed with LPS for 3 hours, with second stimulations of nigericin, 5 mM ATP, 50 μg/ml PGN, MOI 30 of SA113 ΔoatA, POVPC, or PGPC for 16–22 hours. Red signal corresponds to MitoTracker Red CMXRos. Green signal corresponds to ASC-citrine fusion protein diffusely cytosolic or oligomerized into inflammasome specks.

(B) Enumeration of cells with visible ASC specks that also contain polarized mitochondria as a sign of viability. (n > 25 speck containing cells per condition)

(C) Enumeration of cells with visible ASC specks that also phagocytose zymosan particles as a sign of viability. (n > 28 speck containing cells per condition)

See also Figure S3