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Evaluation of chondrogenic and hypertrophic markers in 4-week pellets (A–D) and alginate beads (E–H). For aggrecan (AGC; A,E), type II collagen (Col II; B,F) and type X collagen (Col X; D,H), red staining indicates the marker of interest; blue staining indicates cell nuclei (DAPI). C,G) Red staining indicates the presence of ALP activity.
