Extended Data Figure 7. Patterns of CD8 infiltration and PD-L1 expression in biopsies of patients with desmoplastic melanoma (DM) and non-desmoplastic cutaneous melanoma (non-DM).

Using cut off of >10% for high CD8 density in either parenchyma or invasive margins and >15% for high PD-L1 expression, five different patterns were identified: A) High CD8 density, high PD-L1 tumour parenchyma > invasive margins. B) High CD8 density, high PD-L1 invasive margins > tumour parenchyma. C) High CD8 density, high PD-L1 in the invasive margins only. D) Low CD8 density, high PD-L1. E) Low CD8 density, low PD-L1 expression. F) Yellow lines delineated the edges of tumour regions determined by positive S100 staining. Green or red lines marked the invasive margins around the tumour edges. All analysis was done with the HALO software (Indica Labs). G) Heat map summary of patterns of CD8 and PD-L1 expression in biopsies of patients with DM and CM based on their response to therapy with anti-PD-1/L1; CB: clinical benefit; PD: progressive disease. Intensity of colour coding indicates number of cases in each category. All calculations were based on the scanned whole tumor images.