Fig. 4.

PKM2 siRNA reversed the phosphorylation of ERK and STAT3 in the spinal cord L4–5 segments induced by CCI. Western blot was performed to evaluate the levels of phosphorylated ERK, STAT3, and AKT in protein extracted from the L4–5 segment of spinal cord in each group. GAPDH was used as an internal control. a Treatment with PKM2 siRNA significantly reduced the pERK/ERK and pSTAT3/STAT3 ratios. *P < 0.05 versus NT siRNA group. ^#P < 0.05 versus naïve group. Four rats per group. b PKM2 siRNA failed to inhibit the phosphorylation of AKT. ^#P < 0.05 versus naïve group. Four rats per group