Fig. 5.

Ablation of GRX2 induces a significant increase in PDH protein levels in liver mitochondria. A. Immunoblot analysis of the levels of OGDH using E₁ subunit anti-serum. Band intensities were quantified by ImageJ. MnSOD served as the loading control. n = 3, mean±SEM. 1-way ANOVA Fisher's LSD post-hoc test. B. Immunoblot analysis of the levels of E₁, E₂, and E₃ subunits using PDH antibody cocktail. MnSOD served as the loading control. Band intensities were quantified by ImageJ. n = 3, mean ± SEM. 1-way ANOVA Fisher's LSD post-hoc test.