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. 2017 Dec 14;15:216–227. doi: 10.1016/j.redox.2017.12.006

Fig. 9.

Fig. 9

Impact of GRX2 deletion on mitochondrial respiration and oxidative phosphorylation capacity. A. Liver mitochondria (0.5 mg/mL) were diluted in 1 mL of respiration buffer in the Oxytherm electrode reaction chamber. Once a stable baseline was reached, the different states of respiration were measured. State 2 respiration was induced by adding pyruvate (10 mM) and malate (2 mM) followed by the induction of state 3 respiration (injection of ADP to a final concentration of 1 mM). State 4 respiration was induced by adding oligomycin (4 µg/mL). Finally, respiration was stopped by the addition of antimycin A (4 µM). n = 3–5, mean ± SEM. 1-way ANOVA Fisher's LSD post-hoc test. B. Cardiac mitochondria (0.1 mg/mL) were diluted in 1 mL of respiration buffer in the Oxytherm electrode reaction chamber. Once a stable baseline was reached, the different states of respiration were measured. State 2 respiration was induced by adding pyruvate (10 mM) and malate (2 mM) followed by the induction of state 3 respiration (injection of ADP to a final concentration of 1 mM). State 4 respiration was induced by adding oligomycin (4 µg/mL). Finally, respiration was stopped by the addition of antimycin A (4 µM). n = 4–5, mean ± SEM. 1-way ANOVA Fisher's LSD post-hoc test.