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. 2018 Jan 18;8:1094. doi: 10.1038/s41598-018-19174-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sensitivity of the rapid immunochromatographic (IC) test kit to ECSA- and Asian-genotype chikungunya virus (CHIKV). A rapid IC kit using mouse anti-CHIKV E1 protein antibodies as tracers was tested against two different CHIKV genotypes: ECSA (CP10 strain) and Asian (ARUBA-15801125 and 15801567). The chart displays the measurement of intensity of the test line through the peak height of the immunochromatogram at three points of CHIKV titer: 1.00E + 06, 1.00E + 05, and 1.00E + 04 PFU/ml. Dark red bars represent CHIKV ECSA (CP10 strain); light red and white bars represent the ARUBA-15801125 and 15801567 strains of Asian genotype, respectively. Bar chart represents mean of intensity (milli-absorbance; mABS) and error bars indicate actual fluctuations of duplicate samples.