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. 2018 Jan 18;8:1094. doi: 10.1038/s41598-018-19174-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Indirect immunofluorescence analysis of CK47 and CK119 labeling of virus-infected cells. ECSA (CP10)- and Asian (ARUBA-15801125)-genotype Chikungunya virus (CHIKV) -infected Vero cells (a) and Sindbis virus (SINV) -infected BHK cells (b) were stained with CK47 or CK119; labeling was detected with Alexa Fluor 488-conjugated secondary antibody. Images are representative of results obtained from two independent experiments and were taken under 10 X objective magnification. Alexa Fluor 488 signals of each photo were quantified by NIS-Elements and were expressed as a mean intensity per pixel. Red, white, and blue bars are signals of ECSA-CHIKV, Asian-CHIKV, and SINV, respectively.