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. 2018 Jan 18;8:1128. doi: 10.1038/s41598-018-19598-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescence intensity of RhoP-treated HeLa cells. Each polymer was added to HeLa cells and incubated for 1 h. The fluorescent intensity was evaluated by flow cytometry and analyzed for 5,000 cells. For the analysis, the fluorescence intensity values were normalized based on the Rho substitution degree of each polymer. [RhoP] = 1.0 mg/mL (A) Effect of the PEG chain length. The substitution degree of PEGMA was approximately the same in all polymers; the molecular weight of PEGMA was 2,000 g/mol (left) and 500 g/mol (right). (B) Effect of the position of Rho. The statistical significance of the difference is indicated by * (p < 0.01) vs sRhoP. (C) Effect of molecular weight of αRhoPs. (inset) Data plotted as a function of the polymer molar concentration.