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. 2018 Jan 18;9:267. doi: 10.1038/s41467-017-02494-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Presynaptic proteasome perturbation blocks presynaptic homeostatic plasticity. a Scheme for treatment of NMJs and representative EPSP and mEPSP traces (Rec: recording). Wild-type NMJs were incubated with saline, 100 µM lactacystin for 15 min or 50 µM MG-132 for 15 min, followed by wash and treatment with saline or 20 µM PhTX for 10 min at 0.3 mM [Ca²⁺]_e. DTS-mutant synapses were treated with saline or 20 µM PhTX for 10 min at 0.3 mM [Ca²⁺]_e. A total of 30 sweeps are shown per cell, with the average displayed in darker color. b Quantification of mEPSP amplitude, EPSP amplitude and quantal content for wild-type synapses treated as described in (a). PhTX treatment induced an increase in quantal content (right). Note that PhTX application after lactacystin or MG-132 treatment completely blocks the homeostatic increase in quantal content (right). Mean ± s.e.m.; n ≥ 6 cells; *p < 0.05; **p < 0.001; ***p < 0.0001; ****p < 0.00001; ns: not significant; ANOVA and Tukey’s multiple comparison tests for comparison of EPSP amplitudes, Student’s t-test for pairwise comparison of mEPSP amplitude and quantal content in PhTX-treated vs. non-PhTX-treated cells. c Quantification of mEPSP amplitude, EPSP amplitude and quantal content of control synapses (elav^C155-Gal4 and 24B-Gal4) and synapses expressing mutant proteasome subunits presynaptically (elav^C155-Gal4;UAS-DTS5/+;UAS-DTS7/+; ‘DTS^pre’) or postsynaptically (UAS-DTS5/+;UAS-DTS7/24B-Gal4; ‘DTS^post’). Synapses were treated with 20 µM PhTX or saline for 10 min at 0.3 mM [Ca²⁺]_e. Note that there is no increase in quantal content upon PhTX treatment after genetic presynaptic proteasome perturbation (DTS^pre), indicating a defect in presynaptic homeostatic plasticity. PhTX treatment after postsynaptic proteasome perturbation potentiates quantal content (right), demonstrating that postsynaptic proteasome mutants do not have a defect in homeostatic plasticity. Mean ± s.e.m.; n ≥ 10 cells; *p < 0.05; **p < 0.001; ***p < 0.0001; ****p < 0.00001; ns: not significant; ANOVA and Tukey’s multiple comparison tests