Fig. 3.

Presynaptic overexpression of the mutant proteasome subunit DTS does not induce apparent changes in synapse morphology. a Immunostainings of a control NMJ (elav^C155-Gal4) and an NMJ expressing two copies of the mutant proteasome subunit DTS5 presynaptically (elav^C155-Gal4;UAS-DTS5; ‘DTS^pre’). Larvae were kept at the permissive temperature (25 °C) throughout development. NMJs were stained for neuronal membrane (aHRP), postsynaptic reticulum (aDLG) and the active zone marker Bruchpilot (aBrp); scale bar overview = 20 µm; scale bar inset = 5 µm. b Quantification of NMJ parameters for the two genotypes shown in (a). Presynaptic DTS overexpression does not result in apparent changes in synapse morphology. Mean ± s.e.m.; n = 24 NMJs; Student’s t-test