Figure 5.

The effect of CO-EtOAc on lipid accumulation with OA is dependent on AMPK in FL83B hepatocytes. (A) Cells were pretreated with 20 µM compound C (an AMPK inhibitor) for 1 h followed by treatment with 600 μM OA for 3 h and then treated with 400 μg/mL of CO-EtOAc for another 21 h. All experimental groups and control groups were exposed to the same concentration of DMSO. Intracellular lipid accumulation was determined by Oil Red O assay. The Oil Red O stained lipid droplets were visualized by light microscope and photographed with a digital camera at 200X magnification. (B) To quantitate Oil Red O content levels, isopropanol was added to each sample shaken at room temperature for 5 min, and samples were measured using a microplate reader at 490 nm. All results are presented as the mean ± SEM of three independent experiments. Values are expressed as a percentage change compared with the control group, which is set as 100%. *p < 0.05 indicated the statistics between OA+ CO-EtOAc and OA+ CO-EtOAc+ compound C group; ^#p < 0.05 versus the OA group.