Figure 3.

The effects of CSF samples collected at post-SAH day 5 on NSC proliferation. (A) The cultured NSCs without treatment (control) or treated with CSF samples collected from patients with NPH or SAH were double immunostained with anti-Ki-67 (green) and anti-nestin (red) antibodies with Hoechst 33258 (blue) staining. Initial head CT scan shows SAH in three patients (Patients 33, 1, and 26) with different severity (SAH volume of 9.9, 24.5, and 43.8 mL, respectively). The PI values in these three patients were 9.0, 22.6, and 45.9, respectively. Scale bar = 50 μm. (B) Comparison of the PI between different groups is shown, including control (n = 6), NPH (n = 6), and SAH (n = 36) groups. ***p < 0.001. (C) Correlation between the SAH volume and PI in 36 patients with SAH. p = 0.025 using linear regression analysis. The red solid line is the regression line and dashed lines are 95% confidence limits