Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 18;8:1062. doi: 10.1038/s41598-018-19455-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Genome analysis of phage Toil. (A) Restriction digest of phage Toil genomic DNA with and without proteinase K treatment. Lane 1, NEB 1 kb molecular weight standard; Lane 2, Untreated DNA; Lane 3, proteinase K treated DNA; Lane 4, HindIII digested DNA; Lane 5, HindIII digested and proteinase treated. White arrows in lane 4 point to restriction fragments from the genomic termini predicted to contain covalently-linked protein; digestion of DNA with proteinase K restores band intensity. Full length gel (Fig. S3) is available in supplementary information. (B) Genome maps of phage Toil, enterobacterial phage PRD1 and Bacillus phage Bam35c. Heavy black lines represent the DNA molecules, with boxes above the lines representing genes transcribed from the forward strand and boxes below the lines representing genes transcribed from the reverse strand. Predicted functions of major genes are annotated: DNA polymerase (DNA pol), LysM domain-containing protein (lysM), lytic transglycosylase used for phage DNA entry (entry SLT), CHAP domain-containing protein (CHAP), receptor-binding protein (rbp), and predicted endolysin (lysA). ORF numbers or gene names are indicated for genes with annotated functions. Black arrows on the phage Toil map represent the positions of HindIII sites.