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. 2018 Jan 18;8:1151. doi: 10.1038/s41598-018-19384-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Increased migration and invasion of NSF sublines. (A) For assessment of migration a transwell migration assay was performed and the number of migrated cells was determined after 20 h. In case of HCT116 and DLD-1 cells eight transwell inserts were evaluated; for SW620 cells n = 4. (B) A transwell invasion assay was used and cells invaded through collagen I covered membranes were counted after 24 hours; n = 4 for each individual experiment. Boxes represent the interquartile range; the horizontal line in the box indicates the median; whiskers extend to min and max. P values are indicated. (C) Representative phase contrast pictures of colonies grown from single cells seeded into collagen I gels (CG). Pictures were taken after 6 days (HCT116 and SW620 lines) and 13 days (DLD-1 lines). White asterisks indicate single cells or invasive structures protruding into the matrix. Scale bars: 50 µm.