Table 2.

Discriminatory frequencies (cm⁻¹) from PCA-LDA and associated biomolecular assignments from the intra-retinal layer comparison (between PRL, OPL, INL, and IPL from Akita/+ mice) at 6 weeks, 12 weeks, 6 months, and 10 months of age. The biomolecular assignments acquired from references^56–59 PRL, Photoreceptor retinal layer; OPL, Outer plexiform layer; INL, Inner nuclear layer; IPL, Inner plexiform layer.

6-week-old	12-week-old	6-month-old	10-month-old	Molecular Assignment
1739	1741	1739	1735	Lipids: C=O ester in phospholipids
		1710		Base pair carbonyl (C=O), nucleic acids, DNA, RNA, oxidation of cellular lipids, fatty acids
			1701	Lipids: C=O ester
1685	1681			Proteins: Amide I- turns or antiparallel β-sheet structure (mainly C-O stretching)
1654	1656	1654		Proteins: Amide I- α-helical structure
	1633		1631	Proteins: Amide I: β-sheet structure
1623				Proteins: Amide I- aggregated strand structures (mainly C-O stretching)
	1606	1608		Proteins: Amide I-aggregated strands
			1583	Proteins: Amide II- mainly N=H bending, ring C-C stretch of phenyl
	1560	1550		Proteins: Amide II- mainly N-H bending
1529	1525		1523	Proteins: Amide II- mainly C=N, C=C stretching
			1407	Lipids: C-H deformation
			1296	Proteins: Amide III, mainly N-H bending
		1267		Proteins: Amide III, mainly N-H bending
1236	1222	1220	1224	Nucleic acids: phosphates: νasym PO2−
		1151		C-O, C-C stretching, C-O-H, C-O-C deformation of carbohydrates/ glycogen
	1135			C-O, C-C stretching, RNA ribose
			1120	C-O stretching, RNA ribose
1085	1083	1085		Nucleic acids: phosphates: νsym PO2−
	1049	1051		C–O stretching, deoxyribose/ribose DNA, RNA
			1039	C–O stretching, ribose
			1020	C-O, C-C stretching, DNA, glycogen,
	995			C-O ribose, C-C, RNA
		987		Nucleic acids and proteins, protein phosphorylation, OCH3 (polysaccharides-cellulose)
964	964	964	964	Nucleic acids, symmetric PO4− stretching, (DNA) and deoxyribose; phosphate skeletal motions