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. 2018 Jan 18;9:272. doi: 10.1038/s41467-017-02677-9

Fig. 8.

Fig. 8

FGF21 promotes M2 macrophage polarization specifically in SAT. a–c After 8-week HFD induction and 4 weeks of treatment by vehicle or physiologically relevant dose of rmFGF21 (0.1 mg kg−1 day−1), another cohort of WT + Vehicle, FGF21KO + Vehicle and FGF21KO + rmFGF21 mice were killed for flow cytometry and other following experiments. a Flow cytometry analysis for total, M1 and M2 macrophages in SVF of SAT. b Representative plot chats of flow cytometry analyzing M1 and M2 macrophages after gating F4/80+ cells in SAT. M2 macrophages were defined as F4/80+cd11clowcd206high and M1 macrophages were defined as F4/80+cd11chighcd206low. The gating strategy is described in Supplementary Fig. 6a. c RT-qPCR analysis for mRNA expression levels of F4/80, M1 and M2 macrophages related genes. n = 6. These results were reproduced in two independent experiments. (d–f) At 18th day after fat transplantation, another cohort of WT Sham, KO Sham, KO→KO and WT→KO mice were killed for flow cytometry and other following experiments. d Flow cytometry analysis for total, M1 and M2 macrophages in SVF of SAT. e Representative plot chats of flow cytometry analyzing M1 and M2 macrophages in SAT. f RT-qPCR analysis for mRNA expression levels of F4/80, M1 and M2 macrophages related genes. n = 6. These results were reproduced in two independent experiments. g Insulin-stimulated glucose uptake in adipocytes chronically treated with interleukin (IL)-10 and tumor necrosis factor (TNF)-α. Differentiated SVF adipocytes from WT and FGF21KO mice were treated with low-dose TNFα (3 ng ml−1) for 72 h in the presence or absence of IL10 (20 ng ml−1). Without (white bars) or with insulin (black bars) (100 nm) stimulation, 2-DG uptake was assessed. n = 6 wells. These results were reproduced in three independent experiments. Data are presented as mean ± s.e.m. Significance was determined by one-way ANOVA with Bonferroni multiple-comparison analysis. *P < 0.05, **P < 0.01