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. 2018 Jan 18;9:274. doi: 10.1038/s41467-017-02713-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Structure of the FrdA-SdhE assembly intermediate. a Two orthogonal views of the E. coli FrdA-SdhE assembly intermediate; FrdA (cyan), SdhE assembly factor (green) FAD (orange sticks), and malonate (yellow sticks). The boxed region is highlighted in the inset. The bottom view is looking from the membrane through the top of the complex. b Structure of the assembled E. coli FrdABCD complex (PDB entry 3P4P²¹) shown from the same view; FrdA (gray), FrdB (magenta), FrdC (yellow), and FrdD (blue). The side-by-side comparison of the FrdA-SdhE assembly intermediate and mature FrdABCD shows that the SdhE subunit binds to the same surface in the unassembled complex as the FrdB subunit does in the assembled complex. c Surface representation of the FrdA subunit with the view identical to the bottom panel of a. The binding sites for SdhE (green) and FrdB (magenta) are shown as surfaces and use the same bifunctional binding site. d Overlay of the flavin-binding domains of the FrdA subunit from the FrdA-SdhE assembly intermediate (cyan) and the FrdABCD complex (gray). A rotation of 10.8° is observed in the capping domain of the malonate-bound assembly intermediate when compared to assembled FrdABCD