Table 2.

Effect of SdhE on the kinetic parameters of isolated FrdA and SdhA subunits

		K d fumarate	K d malonate	K d SdhE	IC50SdhE
		(µM)	(µM)	(µM)	(µM)
FrdA		150 ± 15	26 ± 3	0.7 ± 0.1	1.2 ± 0.1a
FrdA+SdhE		70 ± 7	180 ± 20
SdhA		242 ± 16	11 ± 1	1.5 ± 0.2	1.5 ± 0.2a
SdhA+SdhE		64 ± 4	60 ± 5

^aThe IC₅₀ for SdhE inhibition of FrdA and SdhA activity was monitored by inhibition of succinate:DCIP reductase reaction of the flavoproteins in the presence of SdhE